Methylation detection oligonucleotide microarray analysis: a high-resolution method for detection of CpG island methylation.

2.50
Hdl Handle:
http://hdl.handle.net/10143/93136
Title:
Methylation detection oligonucleotide microarray analysis: a high-resolution method for detection of CpG island methylation.
Authors:
Kamalakaran, Sitharthan; Kendall, Jude; Zhao, Xiaoyue; Tang, Chunlao; Khan, Sohail; Ravi, Kandasamy; Auletta, Theresa; Riggs, Michael; Wang, Yun; Helland, Aslaug; Naume, Bjørn; Dimitrova, Nevenka; Børresen-Dale, Anne-Lise; Hicks, Jim; Lucito, Robert
Citation:
Nucleic acids research 2009, 37 (12):e89
Additional Links:
http://nar.oxfordjournals.org/cgi/content/full/37/12/e89

Full metadata record

DC FieldValue Language
dc.contributor.authorKamalakaran, Sitharthanen
dc.contributor.authorKendall, Judeen
dc.contributor.authorZhao, Xiaoyueen
dc.contributor.authorTang, Chunlaoen
dc.contributor.authorKhan, Sohailen
dc.contributor.authorRavi, Kandasamyen
dc.contributor.authorAuletta, Theresaen
dc.contributor.authorRiggs, Michaelen
dc.contributor.authorWang, Yunen
dc.contributor.authorHelland, Aslaugen
dc.contributor.authorNaume, Bjørnen
dc.contributor.authorDimitrova, Nevenkaen
dc.contributor.authorBørresen-Dale, Anne-Liseen
dc.contributor.authorHicks, Jimen
dc.contributor.authorLucito, Roberten
dc.date.accessioned2010-02-26T10:02:42Z-
dc.date.available2010-02-26T10:02:42Z-
dc.date.issued2009-07-
dc.identifier.citationNucleic acids research 2009, 37 (12):e89en
dc.identifier.issn1362-4962-
dc.identifier.pmid19474344-
dc.identifier.doi10.1093/nar/gkp413-
dc.identifier.urihttp://hdl.handle.net/10143/93136-
dc.description.abstractMethylation of CpG islands associated with genes can affect the expression of the proximal gene, and methylation of non-associated CpG islands correlates to genomic instability. This epigenetic modification has been shown to be important in many pathologies, from development and disease to cancer. We report the development of a novel high-resolution microarray that detects the methylation status of over 25,000 CpG islands in the human genome. Experiments were performed to demonstrate low system noise in the methodology and that the array probes have a high signal to noise ratio. Methylation measurements between different cell lines were validated demonstrating the accuracy of measurement. We then identified alterations in CpG islands, both those associated with gene promoters, as well as non-promoter-associated islands in a set of breast and ovarian tumors. We demonstrate that this methodology accurately identifies methylation profiles in cancer and in principle it can differentiate any CpG methylation alterations and can be adapted to analyze other species.en
dc.language.isoenen
dc.relation.urlhttp://nar.oxfordjournals.org/cgi/content/full/37/12/e89en
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Medisinsk genetikk: 714en
dc.subject.meshCell Lineen
dc.subject.meshCpG Islandsen
dc.subject.meshDNA Methylationen
dc.subject.meshGenes, Neoplasmen
dc.subject.meshGenome, Humanen
dc.subject.meshHumansen
dc.subject.meshOligonucleotide Array Sequence Analysisen
dc.titleMethylation detection oligonucleotide microarray analysis: a high-resolution method for detection of CpG island methylation.en
dc.typeJournal articleen
dc.typepeer revieweden
dc.contributor.departmentPhilips Research North America, Briarcliff Manor, NY 10510, USA.en
dc.identifier.journalNucleic acids researchen
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