Molecular Requirements for Sorting of the Chemokine Interleukin-8/CXCL8 to Endothelial Weibel-Palade Bodies.

2.50
Hdl Handle:
http://hdl.handle.net/10143/79733
Title:
Molecular Requirements for Sorting of the Chemokine Interleukin-8/CXCL8 to Endothelial Weibel-Palade Bodies.
Authors:
Hol, Johanna; Küchler, Axel M; Johansen, Finn-Eirik; Dalhus, Bjørn; Haraldsen, Guttorm; Oynebråten, Inger
Citation:
The Journal of biological chemistry. 2009, 284 (35):23532-9

Full metadata record

DC FieldValue Language
dc.contributor.authorHol, Johanna-
dc.contributor.authorKüchler, Axel M-
dc.contributor.authorJohansen, Finn-Eirik-
dc.contributor.authorDalhus, Bjørn-
dc.contributor.authorHaraldsen, Guttorm-
dc.contributor.authorOynebråten, Inger-
dc.date.accessioned2009-09-04T08:29:34Z-
dc.date.available2009-09-04T08:29:34Z-
dc.date.issued2009-08-28-
dc.identifier.citationThe Journal of biological chemistry. 2009, 284 (35):23532-9en
dc.identifier.issn0021-9258-
dc.identifier.pmid19578117-
dc.identifier.doi10.1074/jbc.M900874200-
dc.identifier.urihttp://hdl.handle.net/10143/79733-
dc.description.abstractSorting of proteins to Weibel-Palade bodies (WPB) of endothelial cells allows rapid regulated secretion of leukocyte-recruiting P-selectin and chemokines as well as procoagulant von Willebrand factor (VWF). Here we show by domain swap studies that the exposed aspartic acid in loop 2 (Ser(44)-Asp(45)-Gly(46)) of the CXC chemokine interleukin (IL)-8 is crucial for targeting to WPB. Loop 2 also governs sorting of chemokines to alpha-granules of platelets, but the fingerprint of the loop 2 of these chemokines differs from that of IL-8. On the other hand, loop 2 of IL-8 closely resembles a surface-exposed sequence of the VWF propeptide, the region of VWF that directs sorting of the protein to WPB. We conclude that loop 2 of IL-8 constitutes a critical signal for sorting to WPB and propose a general role for this loop in the sorting of chemokines to compartments of regulated secretion.en
dc.language.isoenen
dc.subjectMedisinsk mikrobiologien
dc.titleMolecular Requirements for Sorting of the Chemokine Interleukin-8/CXCL8 to Endothelial Weibel-Palade Bodies.en
dc.typeJournal articleen
dc.typepeer revieweden
dc.contributor.departmentFrom the Institute and.en
dc.identifier.journalJournal of biological chemistryen
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