A piglet model for detection of hypoxic-ischemic brain injury with magnetic resonance imaging.

2.50
Hdl Handle:
http://hdl.handle.net/10143/57273
Title:
A piglet model for detection of hypoxic-ischemic brain injury with magnetic resonance imaging.
Authors:
Munkeby, Berit Holthe; De Lange, Charlotte; Emblem, Kyrre Eeg; Bjørnerud, Atle; Kro, Grete A B; Andresen, Jannicke; Winther-Larssen, Eldrid H; Løberg, Else Marit; Hald, John K
Citation:
Acta radiologica 2008 Nov;49(9):1049-57
Additional Links:
http://www.informaworld.com/smpp/content~db=all?content=10.1080/02841850802334224

Full metadata record

DC FieldValue Language
dc.contributor.authorMunkeby, Berit Holthe-
dc.contributor.authorDe Lange, Charlotte-
dc.contributor.authorEmblem, Kyrre Eeg-
dc.contributor.authorBjørnerud, Atle-
dc.contributor.authorKro, Grete A B-
dc.contributor.authorAndresen, Jannicke-
dc.contributor.authorWinther-Larssen, Eldrid H-
dc.contributor.authorLøberg, Else Marit-
dc.contributor.authorHald, John K-
dc.date.accessioned2009-03-26T12:59:07Z-
dc.date.available2009-03-26T12:59:07Z-
dc.date.issued2008-11-
dc.identifier.citationActa radiologica 2008 Nov;49(9):1049-57en
dc.identifier.issn1600-0455-
dc.identifier.pmid18720081-
dc.identifier.doi10.1080/02841850802334224-
dc.identifier.urihttp://hdl.handle.net/10143/57273-
dc.description.abstractBACKGROUND: Early detection of hypoxic-ischemic (HI) injury in the asphyxic newborn is important because present prognostic factors are inadequate. Furthermore, therapeutic interventions may have additional benefit if initiated in time. PURPOSE: To assess whether the use of a combined protocol including conventional magnetic resonance imaging (MRI), diffusion-weighted imaging (DWI), diffusion tensor imaging (DTI), and proton MR spectroscopy (MRS) could detect pathological findings in a piglet model 7 hours after HI. MATERIAL AND METHODS: Ten piglets were submitted to HI for 30 min followed by reoxygenation with 21% O2 for 7 hours. MRI at 1.5T was done prior to and 7 hours after the HI. Single-voxel proton MRS was performed, and apparent diffusion coefficient (ADC) and fractional anisotropy (FA) were measured in the basal ganglia. MRS identified N-acetylaspartate (NAA), choline (Cho), creatine (Cr), and lactate (Lac). Histology and microtubule-associated protein 2 (MAP-2) staining was performed in the basal ganglia at the end of the experiment. RESULTS: Compared to baseline, ADC, NAA/Cho, and NAA/Cr were significantly reduced after 7 hours (P<0.001, P=0.01, and P=0.05, respectively) and FA values were increased (P<0.025). The ratios of Lac/Cho and Lac/NAA were significantly higher after 7 hours compared to baseline (P<0.001). Presence of necrosis correlated well with reduced ADC (R(S)=0.91) and presence of Lac (R(S)=0.80). Histology and MAP-2 staining showed more than 90% necrosis in eight piglets, 60% in one piglet, and no necrosis in one piglet. CONCLUSION: Diffusion MRI and proton MRS can detect HI injury in the piglet brain 7 hours after hypoxia. DWI and MRS can be used to give useful prognostic information. This piglet model may potentially be used to mimic clinical situations and is suitable for further research investigating HI injury.en
dc.language.isoenen
dc.relation.urlhttp://www.informaworld.com/smpp/content~db=all?content=10.1080/02841850802334224en
dc.subjectVDP::Medisinske Fag: 700::Klinisk medisinske fag: 750::Radiologi og bildediagnostikk: 763en
dc.subject.meshAnimalsen
dc.subject.meshAnimals, Newbornen
dc.subject.meshAspartic Aciden
dc.subject.meshBasal Gangliaen
dc.subject.meshBrainen
dc.subject.meshBrain Chemistryen
dc.subject.meshCholineen
dc.subject.meshCreatineen
dc.subject.meshDiffusion Magnetic Resonance Imagingen
dc.subject.meshDisease Models, Animalen
dc.subject.meshHypoxia-Ischemia, Brainen
dc.subject.meshLactic Aciden
dc.subject.meshMagnetic Resonance Imagingen
dc.subject.meshMagnetic Resonance Spectroscopyen
dc.subject.meshMicrotubule-Associated Proteinsen
dc.subject.meshSwineen
dc.titleA piglet model for detection of hypoxic-ischemic brain injury with magnetic resonance imaging.en
dc.typeJournal articleen
dc.typepeer revieweden
dc.contributor.departmentDepartment of Paediatric Research, Institute for Surgical Research, Rikshospitalet University Hospital, Oslo, Norway.en
dc.identifier.journalActa radiologica (Stockholm, Sweden : 1987)en
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