Epidermal growth factor receptor levels are reduced in mice with targeted disruption of the protein kinase A catalytic subunit.

2.50
Hdl Handle:
http://hdl.handle.net/10143/54533
Title:
Epidermal growth factor receptor levels are reduced in mice with targeted disruption of the protein kinase A catalytic subunit.
Authors:
Oksvold, Morten P; Funderud, Ane; Kvissel, Anne-Katrine; Skarpen, Ellen; Henanger, Heidi; Huitfeldt, Henrik S; Skålhegg, Bjørn S; Ørstavik, Sigurd
Citation:
BMC cell biology 2008, 9:16
Additional Links:
http://www.biomedcentral.com/1471-2121/9/16

Full metadata record

DC FieldValue Language
dc.contributor.authorOksvold, Morten P-
dc.contributor.authorFunderud, Ane-
dc.contributor.authorKvissel, Anne-Katrine-
dc.contributor.authorSkarpen, Ellen-
dc.contributor.authorHenanger, Heidi-
dc.contributor.authorHuitfeldt, Henrik S-
dc.contributor.authorSkålhegg, Bjørn S-
dc.contributor.authorØrstavik, Sigurd-
dc.date.accessioned2009-03-12T10:03:50Z-
dc.date.available2009-03-12T10:03:50Z-
dc.date.issued2008-04-01-
dc.identifier.citationBMC cell biology 2008, 9:16en
dc.identifier.issn1471-2121-
dc.identifier.pmid18380891-
dc.identifier.doi10.1186/1471-2121-9-16-
dc.identifier.urihttp://hdl.handle.net/10143/54533-
dc.description.abstractBACKGROUND: Epidermal Growth Factor Receptor (EGFR) is a key target molecule in current treatment of several neoplastic diseases. Hence, in order to develop and improve current drugs targeting EGFR signalling, an accurate understanding of how this signalling pathway is regulated is required. It has recently been demonstrated that inhibition of cAMP-dependent protein kinase (PKA) induces a ligand-independent internalization of EGFR. Cyclic-AMP-dependent protein kinase consists of a regulatory dimer bound to two catalytic subunits. RESULTS: We have investigated the effect on EGFR levels after ablating the two catalytic subunits, Calpha and Cbeta in two different models. The first model used targeted disruption of either Calpha or Cbeta in mice whereas the second model used Calpha and Cbeta RNA interference in HeLa cells. In both models we observed a significant reduction of EGFR expression at the protein but not mRNA level. CONCLUSION: Our results suggest that PKA may represent a target that when manipulated can maintain EGFR protein levels at the single cell level as well as in intact animals.en
dc.language.isoenen
dc.relation.urlhttp://www.biomedcentral.com/1471-2121/9/16en
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Medisinsk molekylærbiologi: 711en
dc.subject.meshAnimalsen
dc.subject.meshCyclic AMP-Dependent Protein Kinase Catalytic Subunitsen
dc.subject.meshDown-Regulationen
dc.subject.meshEpidermal Growth Factoren
dc.subject.meshGene Expression Regulation, Enzymologicen
dc.subject.meshHela Cellsen
dc.subject.meshHumansen
dc.subject.meshMiceen
dc.subject.meshMice, Knockouten
dc.subject.meshRNA Interferenceen
dc.subject.meshRNA, Messengeren
dc.subject.meshReceptor, Epidermal Growth Factoren
dc.subject.meshSignal Transductionen
dc.titleEpidermal growth factor receptor levels are reduced in mice with targeted disruption of the protein kinase A catalytic subunit.en
dc.typeJournal articleen
dc.typepeer revieweden
dc.contributor.departmentInstitute of Pathology, Rikshospitalet University Hospital, University of Oslo, Norway. moroks@rr-research.noen
dc.identifier.journalBMC cell biologyen
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