Identification of differentially expressed genes in ileal Peyer's patch of scrapie-infected sheep using RNA arbitrarily primed PCR.

2.50
Hdl Handle:
http://hdl.handle.net/10143/29592
Title:
Identification of differentially expressed genes in ileal Peyer's patch of scrapie-infected sheep using RNA arbitrarily primed PCR.
Authors:
Austbø, Lars; Kampmann, Andreas; Müller-Ladner, Ulf; Neumann, Elena; Olsaker, Ingrid; Skretting, Grethe
Citation:
BMC veterinary research 2008, 4:12

Full metadata record

DC FieldValue Language
dc.contributor.authorAustbø, Lars-
dc.contributor.authorKampmann, Andreas-
dc.contributor.authorMüller-Ladner, Ulf-
dc.contributor.authorNeumann, Elena-
dc.contributor.authorOlsaker, Ingrid-
dc.contributor.authorSkretting, Grethe-
dc.date.accessioned2008-06-06T08:57:19Z-
dc.date.available2008-06-06T08:57:19Z-
dc.date.issued2008-
dc.identifier.citationBMC veterinary research 2008, 4:12en
dc.identifier.issn1746-6148-
dc.identifier.pmid18373840-
dc.identifier.doi10.1186/1746-6148-4-12-
dc.identifier.urihttp://hdl.handle.net/10143/29592-
dc.description.abstractBACKGROUND: In scrapie and prion diseases, the knowledge concerning genes involved in host response during the early infection period in the lymphoid tissues, still remains limited. In the present study, we have examined differential gene expression in ileal Peyer's patches and in laser microdissected follicles of sheep infected with scrapie. METHODS: Ileal Peyer's patches and laser microdissected follicles were of scrapie and control lambs with susceptible genotypes for classical scrapie. Potential regulated genes were found using RNA arbitrarily primed polymerase chain reaction (RAP-PCR) and fingerprinting. The differentially expressed genes were confirmed using real-time RT-PCR. RESULTS: The expression of three genes (MAPRE3, LOC729073 and DNAJC3), were found to be significantly altered in scrapie infected lambs (P < 0.05). CONCLUSION: The three genes have not previously been associated with prion diseases and are interesting as they may reflect biological processes involved in the molecular pathogenesis of prion diseases.en
dc.language.isoenen
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Medisinsk genetikk: 714en
dc.subject.meshAnimalsen
dc.subject.meshGene Expression Profilingen
dc.subject.meshGene Expression Regulationen
dc.subject.meshIleumen
dc.subject.meshPeyer's Patchesen
dc.subject.meshPolymerase Chain Reactionen
dc.subject.meshRNAen
dc.subject.meshScrapieen
dc.subject.meshSheepen
dc.titleIdentification of differentially expressed genes in ileal Peyer's patch of scrapie-infected sheep using RNA arbitrarily primed PCR.en
dc.typeJournal articleen
dc.typepeer revieweden
dc.contributor.departmentDepartment of Basic Sciences and Aquatic Medicine, Norwegian School of Veterinary Science, P.O. Box 8146 Dep., N-0033, Oslo, Norway. lars.austbo@veths.noen
dc.contributor.departmentUllevaal University Hospitalen
dc.identifier.journalBMC veterinary researchen
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