Resuscitation of newborn piglets. short-term influence of FiO2 on matrix metalloproteinases, caspase-3 and BDNF.

2.50
Hdl Handle:
http://hdl.handle.net/10143/142909
Title:
Resuscitation of newborn piglets. short-term influence of FiO2 on matrix metalloproteinases, caspase-3 and BDNF.
Authors:
Solberg, Rønnaug; Løberg, Else Marit; Andresen, Jannicke H; Wright, Marianne S; Charrat, Eliane; Khrestchatisky, Michel; Rivera, Santiago; Saugstad, Ola Didrik
Citation:
Resuscitation of newborn piglets. short-term influence of FiO2 on matrix metalloproteinases, caspase-3 and BDNF. 2010, 5 (12):e14261 PLoS ONE

Full metadata record

DC FieldValue Language
dc.contributor.authorSolberg, Rønnaugen
dc.contributor.authorLøberg, Else Mariten
dc.contributor.authorAndresen, Jannicke Hen
dc.contributor.authorWright, Marianne Sen
dc.contributor.authorCharrat, Elianeen
dc.contributor.authorKhrestchatisky, Michelen
dc.contributor.authorRivera, Santiagoen
dc.contributor.authorSaugstad, Ola Didriken
dc.date.accessioned2011-09-22T07:46:16Z-
dc.date.available2011-09-22T07:46:16Z-
dc.date.issued2010-
dc.identifier.citationResuscitation of newborn piglets. short-term influence of FiO2 on matrix metalloproteinases, caspase-3 and BDNF. 2010, 5 (12):e14261 PLoS ONEen
dc.identifier.issn1932-6203-
dc.identifier.pmid21151608-
dc.identifier.doi10.1371/journal.pone.0014261-
dc.identifier.urihttp://hdl.handle.net/10143/142909-
dc.description.abstractPerinatal hypoxia-ischemia is a major cause of mortality and cerebral morbidity, and using oxygen during newborn resuscitation may further harm the brain. The aim was to examine how supplementary oxygen used for newborn resuscitation would influence early brain tissue injury, cell death and repair processes and the regulation of genes related to apoptosis, neurodegeneration and neuroprotection.en
dc.description.abstractAnesthetized newborn piglets were subjected to global hypoxia and then randomly assigned to resuscitation with 21%, 40% or 100% O(2) for 30 min and followed for 9 h. An additional group received 100% O(2) for 30 min without preceding hypoxia. The left hemisphere was used for histopathology and immunohistochemistry and the right hemisphere was used for in situ zymography in the corpus striatum; gene expression and the activity of various relevant biofactors were measured in the frontal cortex. There was an increase in the net matrix metalloproteinase gelatinolytic activity in the corpus striatum from piglets resuscitated with 100% oxygen vs. 21%. Hematoxylin-eosin (HE) staining revealed no significant changes. Nine hours after oxygen-assisted resuscitation, caspase-3 expression and activity was increased by 30-40% in the 100% O(2) group (n = 9/10) vs. the 21% O(2) group (n = 10; p<0.04), whereas brain-derived neurotrophic factor (BDNF) activity was decreased by 65% p<0.03.en
dc.description.abstractThe use of 100% oxygen for resuscitation resulted in increased potentially harmful proteolytic activities and attenuated BDNF activity when compared with 21%. Although there were no significant changes in short term cell loss, hyperoxia seems to cause an early imbalance between neuroprotective and neurotoxic mechanisms that might compromise the final pathological outcome.en
dc.language.isoenen
dc.publisherhttp://www.plosone.org/en
dc.subjectVDP::Medisinske Fag: 700::Klinisk medisinske fag: 750::Nevrologi: 752en
dc.subject.meshAnimalsen
dc.subject.meshAnimals, Newbornen
dc.subject.meshApoptosisen
dc.subject.meshBrain-Derived Neurotrophic Factoren
dc.subject.meshCaspase 3en
dc.subject.meshCell Deathen
dc.subject.meshCerebellumen
dc.subject.meshCerebral Cortexen
dc.subject.meshCorpus Striatumen
dc.subject.meshHippocampusen
dc.subject.meshMatrix Metalloproteinasesen
dc.subject.meshOxygenen
dc.subject.meshResuscitationen
dc.subject.meshSwineen
dc.subject.meshTime Factorsen
dc.titleResuscitation of newborn piglets. short-term influence of FiO2 on matrix metalloproteinases, caspase-3 and BDNF.en
dc.typeJournal articleen
dc.typepeer revieweden
dc.contributor.departmentDepartment of Paediatric Research, University of Oslo and Oslo University Hospital, Rikshospitalet, Oslo, Norway. ronnaug.solberg@medisin.uio.noen
dc.identifier.journalPloS oneen

Related articles on PubMed

All Items in HeRA are protected by copyright, with all rights reserved, unless otherwise indicated.