Listeria monocytogenes infection affects a subset of Ly49-expressing NK cells in the rat.

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Listeria monocytogenes infection affects a subset of Ly49-expressing NK cells in the rat.
Shegarfi, Hamid; Naper, Christian; Rolstad, Bent; Inngjerdingen, Marit
PloS one 2010, 5 (12)

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dc.contributor.authorShegarfi, Hamiden
dc.contributor.authorNaper, Christianen
dc.contributor.authorRolstad, Benten
dc.contributor.authorInngjerdingen, Mariten
dc.identifier.citationPloS one 2010, 5 (12)en
dc.description.abstractNK cells are protective against certain bacterial and viral infections, and their production of IFN-γ is important for the early innate immune defence against L. monocytogenes. We have previously shown that depletion of NK cells in rats leads to increased bacterial burden upon L. monocytogenes infection, and that a subset of NK cells encompassing the majority of Ly49 receptors (Ly49s3+ NK cells) contributed to this effect. In this study, we have further investigated how the Ly49s3+ NK cell subset is affected by L. monocytogenes infection. We observed an increased percentage of Ly49s3+ NK cells in the spleen and a reduction in the bone marrow within the first 48 hrs of L. monocytogenes infection. Concomitantly, we observed increased expression levels of the inflammatory chemokine receptors CCR5 and CXCR3 by Ly49s3+ bone marrow NK cells, as compared to Ly49s3- NK cells, suggesting involvement of Ly49s3+ NK cells in the early phase of infection. However, NK cell production of IFN-γ was independent of Ly49 receptor expression. Furthermore, we observed increased expression levels of MHC class I molecules on both macrophages and NK cells during the first 48 hrs of infection, paralleled by a reduction in the surface expression of Ly49s3 on NK cells. In conclusion, L. monocytogenes infection modulates the tissue distribution of Ly49s3+ NK cells, and induces increased MHC class I expression and hence reduced surface expression of Ly49 receptors on NK cells. These changes indicate that L. monocytogenes infection may have multiple effects on NK cells in vivo, and suggests the involvement of Ly49-expressing NK cells in the immune responses towards L. monocytogenes.en
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Medisinsk immunologi: 716en
dc.subject.meshBone Marrow Cellsen
dc.subject.meshGene Expression Regulationen
dc.subject.meshHistocompatibility Antigens Class Ien
dc.subject.meshKiller Cells, Naturalen
dc.subject.meshLeukocytes, Mononuclearen
dc.subject.meshListeria monocytogenesen
dc.subject.meshNK Cell Lectin-Like Receptor Subfamily Aen
dc.subject.meshTissue Distributionen
dc.titleListeria monocytogenes infection affects a subset of Ly49-expressing NK cells in the rat.en
dc.typeJournal articleen
dc.typepeer revieweden
dc.contributor.departmentDepartment of Anatomy, Institute of Basic Medical Sciences, University of Oslo, and Institute of Immunology, Oslo University Hospital, Rikshospitalet, Oslo, Norway.en
dc.identifier.journalPloS oneen

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