Tiling array analysis of UV treated Escherichia coli predicts novel differentially expressed small peptides.

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Tiling array analysis of UV treated Escherichia coli predicts novel differentially expressed small peptides.
Thomassen, Gard O S; Weel-Sneve, Ragnhild; Rowe, Alexander D; Booth, James A; Lindvall, Jessica M; Lagesen, Karin; Kristiansen, Knut I; Bjørås, Magnar; Rognes, Torbjørn
PloS one 2010-12-23

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dc.contributor.authorThomassen, Gard O Sen
dc.contributor.authorWeel-Sneve, Ragnhilden
dc.contributor.authorRowe, Alexander Den
dc.contributor.authorBooth, James Aen
dc.contributor.authorLindvall, Jessica Men
dc.contributor.authorLagesen, Karinen
dc.contributor.authorKristiansen, Knut Ien
dc.contributor.authorBjørås, Magnaren
dc.contributor.authorRognes, Torbjørnen
dc.identifier.citationPloS one 2010-12-23en
dc.description.abstractDespite comprehensive investigation, the Escherichia coli SOS response system is not yet fully understood. We have applied custom designed whole genome tiling arrays to measure UV invoked transcriptional changes in E. coli. This study provides a more complete insight into the transcriptome and the UV irradiation response of this microorganism.en
dc.description.abstractWe detected a number of novel differentially expressed transcripts in addition to the expected SOS response genes (such as sulA, recN, uvrA, lexA, umuC and umuD) in the UV treated cells. Several of the differentially expressed transcripts might play important roles in regulation of the cellular response to UV damage. We have predicted 23 novel small peptides from our set of detected non-gene transcripts. Further, three of the predicted peptides were cloned into protein expression vectors to test the biological activity. All three constructs expressed the predicted peptides, in which two of them were highly toxic to the cell. Additionally, a remarkably high overlap with previously in-silico predicted non-coding RNAs (ncRNAs) was detected. Generally we detected a far higher transcriptional activity than the annotation suggests, and these findings correspond with previous transcription mappings from E. coli and other organisms.en
dc.description.abstractHere we demonstrate that the E. coli transcriptome consists of far more transcripts than the present annotation suggests, of which many transcripts seem important to the bacterial stress response. Sequence alignment of promoter regions suggest novel regulatory consensus sequences for some of the upregulated genes. Finally, several of the novel transcripts identified in this study encode putative small peptides, which are biologically active.en
dc.subjectVDP::Medisinske Fag: 700::Klinisk medisinske fag: 750::Infeksjonsmedisin: 776en
dc.subject.meshBase Sequenceen
dc.subject.meshEscherichia colien
dc.subject.meshGene Expression Regulationen
dc.subject.meshMolecular Sequence Dataen
dc.subject.meshOligonucleotide Array Sequence Analysisen
dc.subject.meshOpen Reading Framesen
dc.subject.meshPromoter Regions, Geneticen
dc.subject.meshRNA, Messengeren
dc.subject.meshRNA, Untranslateden
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen
dc.subject.meshSOS Response (Genetics)en
dc.subject.meshTranscription, Geneticen
dc.subject.meshUltraviolet Raysen
dc.titleTiling array analysis of UV treated Escherichia coli predicts novel differentially expressed small peptides.en
dc.typeJournal articleen
dc.typepeer revieweden
dc.contributor.departmentCentre for Molecular Biology and Neuroscience (CMBN) and Department of Microbiology, Rikshospitalet, Oslo University Hospital, Oslo, Norway.en
dc.identifier.journalPloS oneen
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