Mycoplasma contamination revisited: mesenchymal stromal cells harboring Mycoplasma hyorhinis potently inhibit lymphocyte proliferation in vitro.

2.50
Hdl Handle:
http://hdl.handle.net/10143/142369
Title:
Mycoplasma contamination revisited: mesenchymal stromal cells harboring Mycoplasma hyorhinis potently inhibit lymphocyte proliferation in vitro.
Authors:
Zinöcker, Severin; Wang, Meng-Yu; Gaustad, Peter; Kvalheim, Gunnar; Rolstad, Bent; Vaage, John T
Citation:
PloS one 2011, 6 (1):e16005
Additional Links:
http://www.plosone.org/home.action

Full metadata record

DC FieldValue Language
dc.contributor.authorZinöcker, Severinen
dc.contributor.authorWang, Meng-Yuen
dc.contributor.authorGaustad, Peteren
dc.contributor.authorKvalheim, Gunnaren
dc.contributor.authorRolstad, Benten
dc.contributor.authorVaage, John Ten
dc.date.accessioned2011-09-15T07:18:19Z-
dc.date.available2011-09-15T07:18:19Z-
dc.date.issued2011-
dc.identifier.citationPloS one 2011, 6 (1):e16005en
dc.identifier.issn1932-6203-
dc.identifier.pmid21264307-
dc.identifier.doi10.1371/journal.pone.0016005-
dc.identifier.urihttp://hdl.handle.net/10143/142369-
dc.description.abstractMesenchymal stromal cells (MSC) have important immunomodulatory effects that can be exploited in the clinical setting, e.g. in patients suffering from graft-versus-host disease after allogeneic stem cell transplantation. In an experimental animal model, cultures of rat T lymphocytes were stimulated in vitro either with the mitogen Concanavalin A or with irradiated allogeneic cells in mixed lymphocyte reactions, the latter to simulate allo-immunogenic activation of transplanted T cells in vivo. This study investigated the inhibitory effects of rat bone marrow-derived MSC subsequently found to be infected with a common mycoplasma species (Mycoplasma hyorhinis) on T cell activation in vitro and experimental graft-versus-host disease in vivo.en
dc.description.abstractWe found that M. hyorhinis infection increased the anti-proliferative effect of MSC dramatically, as measured by both radiometric and fluorimetric methods. Inhibition could not be explained solely by the well-known ability of mycoplasmas to degrade tritiated thymidine, but likely was the result of rapid dissemination of M. hyorhinis in the lymphocyte culture.en
dc.description.abstractThis study demonstrates the potent inhibitory effect exerted by M. hyorhinis in standard lymphocyte proliferation assays in vitro. MSC are efficient vectors of mycoplasma infection, emphasizing the importance of monitoring cell cultures for contamination.en
dc.language.isoenen
dc.relation.urlhttp://www.plosone.org/home.actionen
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Klinisk kjemi: 725en
dc.subject.meshAnimalsen
dc.subject.meshCell Culture Techniquesen
dc.subject.meshCell Proliferationen
dc.subject.meshCells, Cultureden
dc.subject.meshLymphocyte Activationen
dc.subject.meshMesenchymal Stem Cellsen
dc.subject.meshMycoplasma hyorhinisen
dc.subject.meshRatsen
dc.titleMycoplasma contamination revisited: mesenchymal stromal cells harboring Mycoplasma hyorhinis potently inhibit lymphocyte proliferation in vitro.en
dc.typeJournal articleen
dc.typepeer revieweden
dc.contributor.departmentDepartment of Immunology, Oslo University Hospital, Rikshospitalet and University of Oslo, Oslo, Norway.en
dc.identifier.journalPloS oneen
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